SUMMARY
  • Antibacterial action of an antimicrobial can be assessed by investigating the zone of inhibition.
  • The antibacterial action of Orego-Stim Powder on 4 different bacteria was assessed by analysing the zone of inhibition.
  • The results demonstrate that Orego-Stim Powder provided antimicrobial action against all 4 of the bacteria tested.

 

BACKGROUND
  • The antibacterial efficacy of a product can be easily compared using a zone of inhibition test. This highlights the sensitivity of a known bacteria to multiple products.
  • The larger the zone of inhibition, the more effective the product is against the chosen bacteria.
  • It has been well researched that oregano essential oils can demonstrate antimicrobial activity.
  • Orego-Stim Powder is a high quality eubiotic containing 100% natural oregano essential oil.

 

TRIAL DESIGN

The trial was undertaken by an independent microbiology laboratory in the UK in 2020. A variety of antibiotics and bacterial challenges were selected to account for the most common challenges and associated therapeutic treatment globally. Bacteria were suspended in 20ml of diluent, shaken and the concentration adjusted to contain between 1.5 x 108 – 5.0 x 108 cfu/ml. Plates were prepared in triplicate and incubated at 36ºC ± 1 hour for 24 hours. The zone of inhibition was measured using calipers.

Bacteria selected for use in the trial included:

  • Salmonella typhimurium
  • Escherichia coli (O157)
  • Streptococcus uberis
  • Staphylococcus aureus

 

Antibacterial efficacy of the following treatments against each bacteria were assessed:

  • Lincomycin
  • Amoxicillin
  • Trimethoprim
  • Bacitracin
  • Chloramphenicol
  • Tetracycline
  • Orego-Stim Powder (OSP)

 

RESULTS
  • The antimicrobial activity of Orego-Stim Powder against Salmonella typhimurium and coli was comparable to 4 of the 6 antibiotics tested, producing similar zones of inhibition (Figure 1 and Figure 2).
  • Orego-Stim Powder resulted in a zone of inhibition which was comparable with 5 of the 6 antibiotics tested for antimicrobial efficacy against Staphylococcus aureus, with only Amoxicillin producing a significantly larger zone of inhibition (Figure 3).
  • The zone of inhibition produced by Orego-Stim Powder against Streptococcus uberis was similar to 3 of the 6 antibiotics tested (Figure 4).
  • The results demonstrate that Orego-Stim Powder provided antimicrobial action against all 4 of the bacteria tested.

 

Orego-Stim Antibacterial efficacy of treatments against S. typhimurium and E.coli

Orego-Stim Antibacterial efficacy of treatments against S. aureus and S.uberis

  • Differing letters denote significant differences between treatments (p<0.0001).

 

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