Summary
  • Salkil and Salkil Organic help control levels of bacteria that may impair growth and reduce feed efficiency.
  • Salkil and Salkil Organic ingredients are approved as feed hygiene and feed preservation enhancers.
  • The EU has classified formic acid as a bacterial decontaminating agent (including, but not limited to, Salmonella) to improve feed hygiene (Feed additive Register 2017/940).
Background
  • Anpario acid based eubiotics contain formic acid (EU 1k236), a registered feed hygiene condition enhancer, and propionic acid (E280), a registered feed preservative.
  • This trial was conducted to assess the level of bacterial inhibition in feed.

 

Trial Design

An independent trial was conducted at Abbott Analytical, UK, to test the antibacterial efficacy of Salkil using their standard protocol. 4kg/t Salkil (positive control) and seven inclusion rates of Salkil Organic were assessed, 0 (negative control), 2, 4, 6, 8, 10 and 12 kg/t were added to a standard commercial broiler mash artificially contaminated with Enterobacter cloacae. Bacterial counts (cfu/g) were measured at 4, 24, 48 and 72 hours after application and incubation at 36°C ± 1°C. Full trial method is available upon request.

Results

Graph Showing Results

  • After both 48-hours and 72-hours the antibacterial efficacy of Salkil Organic remained at its optimum at a 2kg/t inclusion. Salkil achieved the same efficacy at a 4kg/t inclusion.
Conclusions
  • Salkil Organic can help control levels of bacteria that may impair animal growth and reduce feed efficiency.
  • The inclusion of Salkil Organic required is related to bacterial load, therefore microbial analysis of feed material is recommended.
  • Salkil Organic is effective in controlling bacterial activity at all recommended inclusion rates and at various contact times.

 

Recommendation

Minimum Salkil and Salkil Organic inclusion and time required to obtain for log 2 reduction (95% reduction).

Enterobacter cloacae 4 hours 24 hours 48 hours 72 hours
Salkil 4 kg/t 4 kg/t 4 kg/t 4 kg/t
Salkil Organic 2 kg/t 2 kg/t 2 kg/t 2 kg/t