SUMMARY
  • Escherichia coli causes diarrhoea in pigs, however Genex Pig has a beneficial effect on the gastrointestinal tract (GIT) environment to support more favourable bacteria, which reduces chances of diarrhoea, thus improving pig growth and efficiency.
  • Pigs fed Genex Pig at 2 kg/tonne demonstrated improved growth rate and FCR compared to the negative control.
  • Genex Pig has been shown to be a cost effective alternative to Tylosin phosphate.

 

BACKGROUND
  • Genex Pig is part of Anpario’s Acid Based Eubiotics (ABE) range and is a blend of liquid formic and propionic acid on a unique mineral delivery system; chosen to ensure maximum efficacy during feed manufacture and throughout the GIT.

 

TRIAL DESIGN

The study was carried out in newly weaned piglets, in a randomised block design and involved 144 pigs from Large White x Landrace sows of a Newsham Hybrid genotype mated to Large White boars. The pigs were individually identified by a coloured and numbered ear tag. Each animal was individually weighed at weaning at 4 weeks of age and were offered a standard commercial starter diet for the first week. At the end of this period, 6 replicates of 6 pigs were randomly allocated to one of the four treatments as below. They were weighed at this point (trial start), after 10 days (mid point) and after 21 days (end point), results are given in Table 1. The four treatments were as follows:

A          Negative control

B          Positive control:    40 g/tonne Tylosin phosphate

C          Treatment 1:         2 kg/tonne Genex Pig

D          Treatment 2:         4 kg/tonne Genex Pig

The second part of the performance trial looked at growing pig performance as an extension to the first study. 36 individually housed pigs from 7 weeks of age (25 kg) were randomly allocated to treatments A, B or C for a further 3 weeks. Results are given in Table 2.

A single pig from 3 replicate pens per treatment were culled at each weighing point (0 days, 10 days and 21 days) of the trial. From these animals, samples of digesta were taken from the upper and mid jejunum, mid ileum and the colon. A fresh faeces sample was taken prior to slaughter. For pigs slaughtered at the 10 and 21 days samples of ileal digesta and faeces were taken, all samples were analysed to determine the bacterial count for the following:

  • Lactobacillus spp
  • Escherichia coli
  • Haemolytic coli

 

RESULTS

 Table 1 – Performance results in newly weaned pigs

A B C D
Negative
Control
Positive Control
Tylosin 40 g/tonne
Genex Pig
2 kg/tonne
Genex Pig  4 kg/tonne
Start weight (kg) 9.72 9.77 9.71 9.74

Live weight gain

(g/d)

0 – 10 days 363 360 372 343
10 – 21 days 568 564 603 564

Feed intake

(g/h/d)

0 – 10 days 395 397 388 382
10 – 21 days 711 720 734 727
FCR 0 – 10 days 1.09 1.10 1.04 1.11
10 – 21 days 1.25 1.28 1.22 1.29

Table 2 – Performance results in grower pigs

A B C
Negative Control Positive Control
Tylosin 40 g/tonne
Genex Pig
2 kg/tonne
Start weight (kg) 27.1 27.4 26.4
Final weight (kg) 41.0 43.4 42.4
Live weight gain (g/day) 662 762 762
Feed intake (kg/day) 1.38 1.50 1.44
FCR 2.08 1.97 1.89
MICROBIOLOGICAL RESULTS

No significant differences were found between any of the sites or any of the groups at the start of the trial.

Day 10 – Results

There were no statistically significant differences at any sites except for the feed treated with 4 kg/tonne Genex Pig in the colon where the level was higher than the negative control.

Lactobacillus spp. There were no statistically significant differences. However, the feed treated with Genex Pig at both levels did increase levels at all sites with the 4 kg/tonne giving the highest number.
E. coli The feed treated with Genex Pig at 4 kg/tonne consistently reduced the population of E. coli throughout the intestine. These reductions were statistically significant in the lower jejunum and faeces. The same was seen with feed treated with Genex Pig at the 2 kg/tonne level but with no statistical significance. Tylosin showed reductions in the colon and faeces at statistically significant levels.
Haemolytic E. coli Numbers of this organism were sporadically found and where they were the effect on them was as for E. coli.

Day 21 – Results

Genex Pig at both levels significantly increased levels in the ileum.

Lactobacillus spp. The feed treated with Genex Pig at 4 kg/tonne significantly increased the population in the ileum.
E. coli No differences were seen at any site.
Haemolytic E. coli No differences were seen.
 Lactobacilli/E. coli ratios

The feed treated with Genex Pig at 4 kg/tonne rate and Tylosin gave a statistically significant increase in this ratio in the upper jejunum. This feed treatment level also showed significant increases in this ratio in the colon and faeces. The feed treated    with Genex Pig at 2 kg/tonne also increased this ratio but only reached significance in the faeces.

These differences were only seen at day 10 and were not apparent at day 21.

CONCLUSION
  • The feed treated with 4 kg/tonne Genex Pig increased the Lactobacilli level throughout the gut both at days 10 and 21 when compared to the other three diets.
  • The levels of E. coli were also reduced by this treatment at the 10 day point. However, this was not seen at day 21.
  • The feed treated with 4 kg/tonne of Genex Pig can be beneficial during the post-weaning period.
  • Animals fed Genex Pig at 2 kg/tonne displayed benefits in growth rate and feed conversion ratio (FCR) compared to the other three treatments.
  • The feed treated with 2 kg/tonne Genex Pig has shown improvements over the negative control in both weight gain and feed conversion and in feed conversion over the positive control.
  • The treatment of feed with 2 kg/tonne Genex Pig is cost effective when compared to that of 40 g/tonne Tylosin phosphate and the improved performance over untreated feeds.